All notable changes to this project will be documented in this file.
The format is based on Keep a Changelog, and this project adheres to Semantic Versioning.
- Updated GENCODE versions:
- Human genome updated from Release 46 to Release 47 (GRCh38.p14).
- Mouse genome updated from Release M35 to Release M36 (GRCm39).
- Initial Release of the Genome Reference Builder Snakemake pipeline.
- Automated genome indexing for:
- Human: GENCODE Release 46 (GRCh38.p14)
- Mouse: GENCODE Release M35 (GRCm39)
- Integration of essential bioinformatics tools:
STAR
(v2.7.11b)RSEM
(v1.3.3)Cell Ranger
(v8.0.1)HISAT2
(v2.2.1)Kallisto
(v0.51.0)Salmon
(v1.10.1)
- GTF filtering step based on the 10x Genomics filtering scheme to ensure consistency between Bulk RNA-seq and Single-cell RNA-seq analyses.
- Configuration file (
config.yaml
) pre-configured with May 2024 GENCODE versions for human and mouse genomes. - Support for both direct execution and SLURM job scheduler submission.
- Comprehensive
README.md
with detailed instructions on installation, configuration, usage, and troubleshooting. - Project structure setup, including directories for configuration, logs, and output.