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[User Story] QC Table #6

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pbiology opened this issue Oct 19, 2023 · 1 comment
Open
2 tasks

[User Story] QC Table #6

pbiology opened this issue Oct 19, 2023 · 1 comment
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User-Story This is a release feature

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@pbiology
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Need

As a clinician I want a table which shows QC metrics for a given sample/case so I can quickly understand if the sample is good enough.

Suggested approach

This could be a table showing at least the following stats.

  • Total bases
  • The number of raw reads
  • Trimmed reads
  • Filtered reads
  • Reads mapped to the host genome (human)
  • Input reads for classifiers
  • Unclassified reads
  • Reads assigned to Homo sapiens, fungi, archaea, bacteria and viruses, respectively.

Can be closed when

  • Endpoint serving up the suggested QC metrics
  • The front end shows the QC metrics in a easy to understand way

Blockers

No response

Anything else?

No response

@pbiology pbiology added the User-Story This is a release feature label Oct 19, 2023
@pbiology pbiology added this to the MVP milestone Oct 19, 2023
@LilyAnderssonLee
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There are some updates regarding QC table:

  • Total reads
  • Total reads after filtering
  • Human mapped reads
  • Human kraken reads
  • % total human reads (mapped+kraken/total reads after filtering)
  • % Bacterial reads (of total reads after filtering)
  • % Virus reads (of total reads after filtering)
  • % Eukaryote reads (of total reads after filtering)
  • % Other reads (of total reads after filtering)
  • % Unclassified reads (of total reads after filtering)
  • %spike reads (of total reads after filtering)
    • Spike Stockholm clinical microbiology using is : Emesvirus zinderi
    • Spikes vary among labs or protocols, so a spikes list can be provided.

The unit for the above QC metrics is pair-end reads. Those QCs can be extracted from MultiQC JSON file.

@moedarrah moedarrah self-assigned this Sep 25, 2024
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