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Check physical coverage
Andrea Telatin edited this page Jan 2, 2020
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11 revisions
The physical coverage can be evaluated when analyzing paired-end or mate paired libraries.
In the repository a simulated mate paired library has been aligned and the sorted BAM file is available in test/mp.bam
.
Command:
covtobed --physical-coverage --min 10 test/mp.bam
Output:
NC_001416.1 40 51 10
NC_001416.1 51 52 11
NC_001416.1 52 56 12
NC_001416.1 56 59 13
NC_001416.1 59 62 14
NC_001416.1 62 63 15
NC_001416.1 63 64 16
NC_001416.1 64 66 17
NC_001416.1 66 73 18
NC_001416.1 73 78 19
Covtobed - Wiki - a simple tool to extract BED coverage tracks from BAM files