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Move advanced flip examples to separate vignette
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Original file line number | Diff line number | Diff line change |
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--- | ||
title: "Advanced flip examples" | ||
author: "Thomas Hackl" | ||
date: "`r format(Sys.Date())`" | ||
vignette: > | ||
%\VignetteIndexEntry{Flip} | ||
%\VignetteEngine{knitr::rmarkdown} | ||
%\VignetteEncoding{UTF-8} | ||
--- | ||
|
||
``` {r} | ||
library(gggenomes) | ||
library(patchwork) | ||
``` | ||
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||
``` {r dpi=36, out.width="720px"} | ||
p <- gggenomes(genes=emale_genes) + | ||
geom_seq(aes(color=strand), arrow=TRUE) + | ||
geom_link(aes(fill=strand)) + | ||
expand_limits(color=c("-")) + | ||
labs(caption="not flipped") | ||
# nothing flipped | ||
p0 <- p %>% add_links(emale_ava) | ||
# flip manually | ||
p1 <- p %>% add_links(emale_ava) %>% | ||
flip(4:6) + labs(caption="manually") | ||
# flip automatically based on genome-genome links | ||
p2 <- p %>% add_links(emale_ava) %>% | ||
sync() + labs(caption="genome alignments") | ||
# flip automatically based on protein-protein links | ||
p3 <- p %>% add_sublinks(emale_prot_ava) %>% | ||
sync() + labs(caption="protein alignments") | ||
# flip automatically based on genes linked implicitly by belonging | ||
# to the same clusters of orthologs (or any grouping of your choice) | ||
p4 <- p %>% add_clusters(emale_cogs) %>% | ||
sync() + labs(caption="shared orthologs") | ||
p0 + p1 + p2 + p3 + p4 + plot_layout(nrow=1, guides="collect") | ||
``` | ||
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||
``` {r dpi=36, out.width="720px"} | ||
# flip seqs inside bins | ||
s0 <- tibble::tibble( | ||
bin_id = c("A", "B", "B", "B", "C", "C", "C"), | ||
seq_id = c("a1","b1","b2","b3","c1","c2","c3"), | ||
length = c(1e4, 6e3, 2e3, 1e3, 3e3, 3e3, 3e3)) | ||
p <- gggenomes(seqs=s0) + | ||
geom_seq(aes(color=bin_id), size=1, arrow = arrow(angle = 30, length = unit(10, "pt"), | ||
ends = "last", type = "open")) + | ||
geom_bin_label() + geom_seq_label() + | ||
expand_limits(color=c("A","B","C")) | ||
p1 <- p %>% flip_seqs(6) | ||
p2 <- p %>% flip_seqs(c2) | ||
p3 <- p %>% flip_seqs(2, .bins = C) | ||
p + p1 + p2 + p3 + plot_layout(nrow=1, guides="collect") | ||
``` | ||
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||
``` {r dpi=36, out.width="720px"} | ||
# fancy flipping using tidyselect::where for dynamic selection | ||
p <- gggenomes(emale_genes,emale_seqs) %>% add_clusters(emale_cogs) + | ||
geom_seq(color="grey70", size=1, arrow = arrow(angle = 30, length = unit(15, "pt"), | ||
ends = "last", type = "open")) + | ||
geom_gene(aes(fill=cluster_id)) | ||
# flip all short seqs - where() applied to .bin_track=seqs | ||
p1 <- p %>% flip(where(~.x$length < 21000)) | ||
# flip all seqs with MCP on "-" - where() applied to .bin_track=genes | ||
p2 <- p %>% flip(where(~any(.x$strand[.x$cluster_id %in% "cog-MCP"] == "-")), .bin_track=genes) | ||
p + p1 + p2 + plot_layout(nrow=1, guides="collect") & theme(legend.position = "bottom") | ||
``` |